Additional file 1: Table S1. of Glucose transporter 4 promotes head and neck squamous cell carcinoma metastasis through the TRIM24-DDX58 axis

Demographic features of HNCC patient cohort. Table S2. GLUT overexpression activated transcription factors and their downstream targets ranked by Z-Score. Table S3. GLUT overexpression inhibited transcription factors and their downstream targets ranked by Z-Score. Table S4. List of TRIM24 downstream genes and their fold changes. Table S5. List of primers and knockdown clones’ information. Table S6. List of candidate probes >2.0-fold change cutoff by GLUT4 vs. control in FaDu cells. Figure S1. Box plot showing the expression of the GLUT family members correlated with the survival rate of the patients in the Petel HNSCC cohort (E-MTAB-1328, n = 89) in the SurvExpress database (HR = 3.37, P = 0.043). Figure S2. Forest plot of GLUT family members and their corresponding hazard ratios, probes and Cox-P values. Figure S3. GLUT4 overexpression model in vitro and in vivo. (A) Cell proliferation rate and (B) tumorigenicity ability in animal model GLUT4-overexpressing FaDu cells. Figure S4. Glucose uptake and lactate production in a panel of HNSCC cell lines. Figure S5. The migration abilities of with or without GLUT4 knockdown combined DDX58 or OASL knockdown in HSC-2 cells. Figure S6. Correlation plot of GLUT4 expression with the (A) OASL or (B) DDX58 RNA level in a clinical cohort (Pearson r = −0.7146, P < 0.001 and Pearson r = −0.6246, P < 0.001, respectively). (DOCX 2697 kb)