Additional file 1: Figure S1. of Successful production of the potato antimicrobial peptide Snakin-1 in baculovirus-infected insect cells and development of specific antibodies

Determination of the antibody titer by immunoblots assays. Purified rSN1 (300 μg per lane) or total potato leaf protein extract (30 mg per lane) were employed as epitope. Serial dilutions at third were made from total polyclonal serum: Lane 1: 1/100 dilution; Lane 2: 1/300 dilution; Lane 3: 1/900 dilution; Lane 4: 1/2700 dilution; Lane 5: 1/8100 dilution; Lane 6: 1/24300 dilution; Lane 7: 1/72900 dilution; Lane 8: 1/218700 dilution. (TIFF 361 kb)