Additional file 1: Figure S1. of Role of P2X7R in the development and progression of pulmonary hypertension

Validation of the MCT plus - pneumonectomy induced PAH model. (A-B) Rats were given a single intraperitoneal injection of 60 mg/kg MCT one week after left pneumonectomy or vehicle, and RV systolic pressure (A) and RV weight (B) were measured 1, 2, 3, or 4 weeks after MCT challenge. (C–F) H&E staining and a-SMA staining of lung tissue sections at 4 weeks after MCT injection. (G) The % medial wall thickness was calculated as [(medial thickness × 2)/external diameter] × 100. Scale bars  = 50 μm. All data are expressed as mean ± SD. n =  6 per group. **p < 0.01 and *p < 0.05. Figure S2 Representative double-immunostained images of P2X7R (Red), co-stained for FITC-CD68, DAPI (Blue) for nuclei and merged images in the P/MCT (A), sham group (B) and negative control (C), Original magnification × 40. Scale bar = 50 μm for all images. (DOCX 1872 kb)