Additional file 1: Figure S1. of Muscle-specific deletion of SOCS3 increases the early inflammatory response but does not affect regeneration after myotoxic injury

Genomic PCR to confirm correct Socs3 deletion. (A) Schematic of Socs3 gene demonstrating floxed regions (LoxP) flanking exon 2 and location of the sequencing primers both prior to and post-Cre excision. (B) Genomic PCR using the primers shown in (A) confirmed the presence of the 288 bp deleted DNA band in muscle but not liver of SOCS3fl/fl MCK-Cre positive and not SOCS3fl/fl MCK-Cre-negative mice. (C) qRT-PCR using primers to detect Socs3 gene expression in RNA extracted from muscle fibers isolated from freeze-dried gastrocnemius muscles of saline or LPS-injected control and SOCS3 MKO mice. Data are expressed as mean ± SEM and compared with a two-way ANOVA and Fisher’s LSD post hoc multiple comparisons test to determine the effect of genotype and LPS injection (n = 2 mice/genotype). ***P < 0.001 compared to uninjured muscle from control mice. (PDF 115 kb)