Additional file 1: Figure S1. of Isocitrate dehydrogenase mutation in Vibrio anguillarum results in virulence attenuation and immunoprotection in rainbow trout (Oncorhynchus mykiss)

Hemolytic activity of various V. anguillarum strains grown on fish blood agar. Colonies grown overnight on LB20 plates were tooth picked onto LB20 + 5% trout blood agar plates. The diameter of the zones of hemolysis were measured after 7 h and 23 h of growth at 27 °C. Between marked strains and M93Sm: * p < 0.05 and ** p < 0.01. Error bars represent 1 standard deviation (PDF 146 kb)