Additional file 1: Figure S1. of Immune response in peripheral axons delays disease progression in SOD1G93A mice

Normalized frequency histograms of the α values computed from all the acquired RP-CARS z-stacks in the four conditions: Ntg vs. SOD1G93A and C57 vs. 129Sv. Figure S2. GFAP and P-ERK expression is reduced in the sciatic nerve of 129Sv_Ntg. Figure S3. H2-K_D (MhcI; red), and β2m (blue) mRNA levels from laser captured MNs of C57Ntg versus 129SvNtg mice. H2-K_D (red), Lmp7 (green) and β2m (blue) and Tap1 (black) longitudinal mRNA levels from laser captured MNs of C57SOD1G93A versus Ntg littermates during the progression of the disease. MHCI immunohistochemistry shows an increased and widely diffuse expression in the ventral grey and white matter of the lumbar spinal cord of C57SOD1G93A mice at disease onset compared to Ntg littermates β2M (green) and LMP7 (red) colocalization in a subset of lumbar spinal MNs (blue, NT) of C57SOD1G93A mice (inset) at the onset compared to Ntg littermates (scale bar: 50 μm; inset scale bar: 20 μm). Representative western blot of LMP7 and β2M expression in protein extracts from longitudinally dissected lumbar ventral spinal cord of C57SOD1G93A mice at the disease onset versus Ntg littermates. Figure S4. β2M is activated in the peripheral nerves of SOD1G93A mice. Figure S5. Real-time PCR for CD8+ co-receptor transcript in the spinal cord of 129SvSOD1G93A mice and Ntg littermates at disease onset. Figure S6. Confocal Z-stack orthogonal projection of a longitudinal section of sciatic nerve of C57SOD1G93A mice showing the co-localization of signals between S100beta (red) and Iba1(green), dapi (blue). RT-qPCR for Ym1, Tnfα, Il6 and Ly6c co-receptor transcript in the sciatic nerve of C57SOD1G93A mice compared to Ntg littermates at disease onset. (PDF 1222 kb)