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Additional file 1: Figure S1. of Diet-induced obesity alters myeloid cell populations in naĂŻve and injured lung
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posted on 2016-03-08, 05:00 authored by Anne Manicone, Keqin Gong, Laura Johnston, Matthew GiannandreaAdhesion Receptor Expression on Bone Marrow Leukocytes at 3 Months on HFD vs. LFD. Bone marrow cells were isolated, counted, and immunostained with anti-murine antibodies to CD45, Ly6G, CD11b, Ly6C, CD62L, ICAM-1, CXCR2, and LFA-1 and analyzed by FACS. MFI for adhesion and chemokine receptors were measured in (A) PMNs and (B) Ly6Chigh monocytes. (A) Among those receptors expressed by these cells, there was no difference in LFA-1, CD62L and CXCR2 levels in HFD vs. LFD PMNs. (B) Similarly, there was no difference in LFA-1 and CD62L expression in Ly6Chigh monocytes. (TIF 1076 kb)