13287_2017_519_MOESM1_ESM.tif (10.68 MB)

Additional file 1: Figure S1. of Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential

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posted on 2017-03-17, 05:00 authored by Yekaterina Galat, Svetlana Dambaeva, Irina Elcheva, Aaruni Khanolkar, Kenneth Beaman, Philip Iannaccone, Vasiliy Galat

showing characterization of iPSC-SR2 and gating strategy for lymphoid cell analysis. (A) Normal karyotype of iPSC-SR2. (B) Pluripotency assessment results of iPSC-SR2 by PluriTest. (C) Gating strategy for identifying various lymphoid cells in differentiating cultures after CHIR99021 induction and OP9 coculture induction. R1 representing CD45+ showing CD15, CD56, CD16, or CD94 expression, while none of these markers are present on cells in R2. (TIF 10931Â kb)

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Hemogenic endothelium Pluripotent stem cells Hematopoietic cells Lymphocytes Glycogen synthase kinase 3 inhibitor

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