Additional file 1: Figure S1. of Cytokine-free directed differentiation of human pluripotent stem cells efficiently produces hemogenic endothelium with lymphoid potential

showing characterization of iPSC-SR2 and gating strategy for lymphoid cell analysis. (A) Normal karyotype of iPSC-SR2. (B) Pluripotency assessment results of iPSC-SR2 by PluriTest. (C) Gating strategy for identifying various lymphoid cells in differentiating cultures after CHIR99021 induction and OP9 coculture induction. R1 representing CD45+ showing CD15, CD56, CD16, or CD94 expression, while none of these markers are present on cells in R2. (TIF 10931 kb)