Additional file 1: Figure S1. of Astroglia acquires a toxic neuroinflammatory role in response to the cerebrospinal fluid from amyotrophic lateral sclerosis patients

Representative phase contrast (a, c–e) and confocal images of the enriched astroglial cultures (b, f–h). The reactive astrocytes display a process bearing morphology (e, h) as compared to the flat morphology adopted by the non-reactive astrocytes (c, f). The intermediate stages show a semi-reactive morphology with a transformation from flat to process bearing one (d, g). The cultures were found to be free of microglia as seen by the presence of GFAP immunoreactivity (red, j) and absence of Iba1 (green, i). In a similar manner, the cultures were found to be ChAT negative (green, l). The cultures were >99 % pure. Scale bars are indicated. (TIF 14438 kb)