Additional file 1: Figure S1. of Alzheimer’s disease-like APP processing in wild-type mice identifies synaptic defects as initial steps of disease progression

APP is processed in sporadic AD cases. Human samples were obtained from late-onset AD cases (Braak 6, Thal 5) and age-matched controls (n = 5 per group). The hippocampus was the studied structure. (A) Western blot analysis of NeuN. (B) Densitometric analyses of western blots showing the expression of human APP in the hippocampus of human controls and AD cases (n = 5 per group). Bars represent means ± SEM, and data were normalized with respect to GAPDH. Statistical analyses were performed with Student’s t-test: **p < 0.01. (C) Densitometric analyses of A, showing the expression of NeuN in the hippocampus of human controls and AD cases (n = 5 per group). Bars represent means ± SEM, and data were normalized with respect to GAPDH. Statistical analyses were performed with Student’s t-test: *p < 0.05. (D) Representation of the APP/NeuN ratio following densitometric analyses of the corresponding western blots (n = 5 per group). Note that APP seems to be processed in sporadic AD cases. Bars represent means ± SEM and data were normalized with respect to GAPDH. Statistical analyses were performed with Student’s t-test: *p < 0.05. (PNG 325 kb)