Additional file 1: Figure S1A. of 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response

3D ‘On-Top’ Cell culture method. A modified version of the ‘3D on-top’ assay reported by Lee et al. [17] was used for analysis of cellular growth in 3D culture and optimised for the cell lines under test here. (DOC 41 kb)