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Additional file 1: Figure S18. of Temporally distinct transcriptional regulation of myocyte dedifferentiation and Myofiber growth during muscle regeneration
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posted on 2017-11-09, 05:00 authored by Keâale Louie, Alfonso Saera-Vila, Phillip Kish, Justin Colacino, Alon KahanaDiagram of the strategy of the present study. RNA was isolated and sequenced from laser microdissected adult zebrafish LR muscle tissue at multiple time points post injury. Transcriptome assembly and DEG identification was performed using the Tuxedo Suite. DEG functional classification was concurrently performed via separate strategies using either gene set enrichment analysis (left: KEGG pathway, GO term analysis [GAGE]) or expression profile-based clustering (right: K-means clustering [cummeRbund] and GO enrichment [Panther]). MO knockdown-based phenotypic screens included both cell proliferation and overall regeneration assays as readouts of DEG functional significance. (TIFF 18214 kb)