Additional file 15: Figure S8. of Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants

Melting curves of PCR amplicons from probiotic strains and bacterial preterm isolates. a Melting curves of PCR amplicons from probiotic strains (Bifidobacterium bifidum and Lactobacillus acidophilus) and bacterial preterm isolates (Enterococcus faecium and Streptococcus infantarius). Primers used to generate these amplicons were 530F-926R targeting region (V4 + V5). Bifidobacterium bifidum displayed the highest melting temperature. b Melting curve of PCR amplicon obtained from a mixed DNA sample (5 ng of Bifidobacterium bifidum, 5 ng of Lactobacillus acidophilus, 5 ng of Enterococcus faecium and 5 ng of Streptococcus infantarius). Primers used to generate these amplicons were 530F-926R targeting region (V4 + V5). Peak name (2) presents a melting temperature (Tm) similar to the melting temperature (Tm) obtained for B. bifidum. (PDF 100 kb)