Additional file 12: of Inhibition of monocyte-like cell extravasation protects from neurodegeneration in DBA/2J glaucoma

Figure S7. Monocytes entering the ONH during glaucoma are platelet bound. ONHs assessed by flow cytometry demonstrate that the majority of monocytes entering the ONH during glaucoma pathogenesis are platelet bound (Fig. 3). To further confirm that monocyte-platelet aggregates were present in the ONH in glaucoma, immunofluorescence using antibodies against CD41 (a platelet marker, green) and CD11b (a monocyte maker, red) was performed in longitudinal sections of D2 and D2-Gpnmb+ ONH tissue (n = 4 / group, see upper right panel for ONH location for all upper and middle panels). The majority of monocytes that enter the ONH during glaucoma in D2 eyes are platelet-bound. APC = allophycocyanin (red), FITC = fluorescein isothiocyanate (green). Scale bar = 100 μm. (TIF 9562 kb)