Additional file 11: Figure S6. of A comparative analysis of nonhost resistance across the two Triticeae crop species wheat and barley

Results of k-means clustering followed by hierarchical clustering on the nonhost-related DEGs of wheat and barley in each pathosystem. In an independent approach to identify co-regulated orthologues, a k-means clustering followed by a hierarchical clustering of individual k-means cluster members was carried out for both cereal species and respective interactions. For this, the normalized signal intensity values of host and nonhost DEGs (Fig. 2b) of the respective pathosystem were median-centered separately for wheat and barley in Genesis software (release 1.8.0, [88]). The median-centered signal values of both species were pooled for each pathosystem to form a single list, and a k-means clustering analysis was done in Genesis with default settings. Individual k-means clusters were then hierarchical clustered in Genesis with Pearson correlation, average linkage settings. To identify co-regulated regulons, sub-clusters with the occurrence of both wheat and barley probes (left clustering image; nodes with sub-clusters highlighted in color) were manually identified and extracted. A zoomed in image of these sub-clusters is shown on the right. Please note the occurrence of both wheat (A_99_) and barley (CUST_) probe IDs in the same node. In order to determine the gene function relation among these co-regulated wheat and barley DEGs, MapMan (3.5.1R2) and BlastN analyses (default parameters, BLASTN 2.2.29+) were carried out. (PDF 3400 kb)