Additional file 10: Figure S7. of Lipocalin2 suppresses metastasis of colorectal cancer by attenuating NF-κB-dependent activation of snail and epithelial mesenchymal transition

Association between LCN2 and NF-ĸB/snail pathway in the indicated cells. (A) Changes of MMP9, p65 and snail expression in RKO-LCN2 cells and quantification of relative mRNA expression of EMT key proteins after treatment with LMB (40nM, 6 h) in RKO-LCN2 cells. (B) Changes of EMT key proteins, p65 expression and quantification of relative mRNA expression of EMT key proteins after transfection with specific siRNA (50nM, 48 h) of NF-ĸBp65 in HT-29-sh-LCN2 cells, (N, nuclear; C, cytoplasm; P-phosphorylated). The grey value ratios of the corresponding proteins/GAPDH were shown. Values shown in real-time PCR assay are the mean ± SD from at least three independent experiments. * P < .05, ** P < .01, *** P < .001. (TIF 4190 kb)