12915_2016_325_MOESM10_ESM.pdf (48.32 kB)
Additional file 10: Figure S5. of Functional characterisation of long intergenic non-coding RNAs through genetic interaction profiling in Saccharomyces cerevisiae
journal contribution
posted on 2016-12-07, 05:00 authored by Dimitris Kyriakou, Emmanouil Stavrou, Panayiota Demosthenous, Georgia Angelidou, Bryan-Joseph San Luis, Charles Boone, Vasilis Promponas, Antonis KirmizisDegradation of telomeric ssDNA by bacterial Exonuclease I. Genomic DNA extracted from four individual sut457Δ strains at passage five was either untreated (-) or digested with bacterial exonuclease I (+), which degrades telomeric overhang DNA in the 3’ to 5’ direction, before being subjected to native southern analysis using a biotinylated probe against telomeric repeats (upper blot). The DNA on the membrane was then denatured with 0.4 N NaOH and re-probed to monitor equal loading (lower blot). (PDF 48 kb)