ac3000122_si_001.pdf (424.12 kB)
Absorption-Mode: The Next Generation of Fourier Transform Mass Spectra
journal contribution
posted on 2012-03-20, 00:00 authored by Yulin Qi, Mark P. Barrow, Huilin Li, Joseph E. Meier, Steve
L. Van Orden, Christopher J. Thompson, Peter B. O’ConnorThe Fourier transform spectrum can be presented in the
absorption-mode
(commonly used in FT-NMR), magnitude-mode (FT-ICR), and power-mode
(engineering applications). As is routinely used in FT-NMR, it is
well-known that the absorption-mode display gives a much narrower
peak shape which greatly improves the spectrum; recently, the successful
solution of the phase equation allowed broadband phase correction
which makes it possible to apply the absorption-mode routinely in
FT-ICR. With the empirical evidence provided herein, it has been confirmed
that in addition to the improvement on resolving power, compared to
the conventional magnitude-mode, the new absorption-mode improves
the signal-to-noise ratio (S/N) of a spectrum by 1.4-fold and can
improve the mass accuracy up to 2-fold with no extra cost in instrumentation.
Therefore, it is worthwhile to apply and promote absorption-mode in
routine FT-ICR experiments.