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Absolute Quantification of Uric Acid in Human Urine Using Surface Enhanced Raman Scattering with the Standard Addition Method
journal contribution
posted on 2017-01-19, 00:00 authored by Chloe Westley, Yun Xu, Baskaran Thilaganathan, Andrew J. Carnell, Nicholas J. Turner, Royston GoodacreHigh
levels of uric acid in urine and serum can be indicative of
hypertension and the pregnancy related condition, preeclampsia. We
have developed a simple, cost-effective, portable surface enhanced
Raman scattering (SERS) approach for the routine analysis of uric
acid at clinically relevant levels in urine patient samples. This
approach, combined with the standard addition method (SAM), allows
for the absolute quantification of uric acid directly in a complex
matrix such as that from human urine. Results are highly comparable
and in very good agreement with HPLC results, with an average <9%
difference in predictions between the two analytical approaches across
all samples analyzed, with SERS demonstrating a 60-fold reduction
in acquisition time compared with HPLC. For the first time, clinical
prepreeclampsia patient samples have been used for quantitative uric
acid detection using a simple, rapid colloidal SERS approach without
the need for complex data analysis.