A novel chloro-substituted pentenamide from the fruiting bodies of <i>Amanita virgineoides</i>

<p>One unusual chloro-substituted pentenamide, (3<i>R</i>)-4-chloro-3-hydroxy-4-pentenamide (<b>1</b>), together with 11 known compounds (<b>2</b>–<b>12</b>) were isolated from the fruiting bodies of <i>Amanita virgineoides</i>. The structure of <b>1</b> including the absolute configuration was characterized by extensive spectroscopic analyses and quantum calculation. Compound <b>1</b> displayed no obvious activity against herpes simplex virus (HSV), human enterovirus 71 (EV71) or coxsackievirus B3 (CVB3).</p>