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A Novel Acetylcholinesterase Biosensor: Core–Shell Magnetic Nanoparticles Incorporating a Conjugated Polymer for the Detection of Organophosphorus Pesticides
journal contribution
posted on 2016-03-09, 00:00 authored by Hurija Dzudzevic Cancar, Saniye Soylemez, Yeliz Akpinar, Melis Kesik, Seza Göker, Gorkem Gunbas, Murvet Volkan, Levent ToppareTo construct a sensing interface,
in the present work, a conjugated
polymer and core–shell magnetic nanoparticle containing biosensor
was constructed for the pesticide analysis. The monomer 4,7-di(furan-2-yl)benzo[c][1,2,5]thiadiazole (FBThF) and core–shell magnetic
nanoparticles were designed and synthesized for fabrication of the
biosensing device. The magnetic nanoparticles were first treated with
silica and then modified using carboxyl groups, which enabled binding
of the biomolecules covalently. For the construction of the proposed
sensor a two-step procedure was performed. First, the poly(FBThF)
was electrochemically generated on the electrode surface. Then, carboxyl
group modified magnetic nanoparticles (f-MNPs) and acetylcholinesterase
(AChE), the model enzyme, were co-immobilized on the polymer-coated
surface. Thereby, a robust and novel surface, conjugated polymer bearing
magnetic nanoparticles with pendant carboxyl groups, was constructed,
which was characterized using Fourier transform infrared spectrometer,
cyclic voltammetry, scanning electron microscopy, and contact angle
measurements. This novel architecture was then applied as an immobilization
platform to detect pesticides. To the best of our knowledge, a sensor
design that combines both conjugated polymer and magnetic nanoparticles
was attempted for the first time, and this approach resulted in improved
biosensor characteristics. Hence, this approach opens a new perspective
in the field of enzyme immobilization and sensing applications. Paraoxon
and trichlorfon were selected as the model toxicants. To obtain best
biosensor performance, optimization studies were performed. Under
optimized conditions, the biosensor in concern revealed a rapid response
(5 s), a low detection limit (6.66 × 10–3 mM),
and high sensitivity (45.01 μA mM–1 cm–2). The KMapp value of poly(FBThF)/f-MNPs/AChE were determined as 0.73 mM. Furthermore,
there was no considerable activity loss for 10 d for poly(FBThF)/f-MNPs/AChE
biofilm.
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Keywords
activity lossimmobilization platformpendant carboxyl groupsnanoparticleoptimization studiesscanning electron microscopyOrganophosphorus Pesticidescyclic voltammetryNovel Acetylcholinesterase Biosensorpesticide analysissensor designbiosensor performancecontact angle measurementselectrode surfacenovel architecturemodel enzymebiosensor characteristicsoptimized conditionspoly0.73 mMbiomolecules covalentlybiosensing devicepolymerConjugated PolymerNanoparticles Incorporatingenzyme immobilizationnovel surfaceKMapp valuecarboxyl groupmodel toxicantscarboxyl groups
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