ATP:ADP antiporter mimics turbo-state.

<p>(<i>A</i>) Overview of the models used in this figure. Model A and D are from <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g001" target="_blank">Figure 1</a>, model A–glyc is model A without glycosomal localization, as described in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi.1003371-Haanstra2" target="_blank">[31]</a>, model A+AAT is model A with an ATP:ADP antiporter. (<i>B–C</i>) Steady-state concentrations of glycosomal Glc-6-P and Fru-1,6-BP are depicted in the various models. (<i>D</i>) Increasing the activity of the ATP:ADP antiporter (V<sub>max,ATP:ADP antiporter</sub>) in model D leads to a high risk of accumulation of hexose phosphates. The green line indicates the concentration of Fru-1,6-BP in the original model of glycolysis (17.2 mM, panel C, model A). Glc<sub>e</sub> in this simulation is 25 mM. (<i>E</i>) Time course simulation of model D at 25 mM Glc<sub>e</sub> and various values for the V<sub>max,ATP:ADP antiporter</sub> parameter. Plotted is the concentration of glycosomal phosphates (ΣP similar as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g002" target="_blank">Figure 2</a>, moiety 5 in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-t002" target="_blank">Table 2</a>). ATP:ADP antiporter activity values below 1 nmol·min<sup>−1</sup>·mg protein<sup>−1</sup> result in depletion of glycosomal phosphates (cf. <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g002" target="_blank">Figure 2</a>). <i>k<sub>TOX</sub></i> = 2 µl·min<sup>−1</sup>·mg protein<sup>−1</sup> in all models. Solid lines indicate medians, shaded areas and error bars show interquartile ranges, as derived from the uncertainty modeling.</p>