Fig 11.tif (3.7 MB)
ASGPR expression by hiPSC- or hESC-derived hepatocytes in 2D or 3D on day 13 of differentiation.
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posted on 2017-12-20, 18:38 authored by Maëlle Lorvellec, Federico Scottoni, Claire Crowley, Rebeca Fiadeiro, Panagiotis Maghsoudlou, Alessandro Filippo Pellegata, Francesca Mazzacuva, Asllan Gjinovci, Anne-Marie Lyne, Justine Zulini, Daniel Little, Olukunbi Mosaku, Deirdre Kelly, Paolo De Coppi, Paul Gissen(a-b) Immunostaining of hepatocyte sinusoidal membrane protein ASGPR in 2D (a) and 3D (b) cultured cells. Nuclei stained with DAPI. The higher magnification images of merged channels in the bottom panel correspond to the delineated lower magnification images in the panel above. Images of 3D cultured cells demonstrate membrane localisation of ASGPR.
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hepatocyte marker albumin2 D cultureshepatocyte marker alpha-fetoprotein2 D conditionshepatocyte-like cells Liver transplantationregenerative medicine applicationsPluripotent Stem Cellscell hepatocyte differentiation3 D cultureorgan regeneration therapiesoptimised 3 D protocolsblood vessel networkmouse decellularised liver scaffold3 Ddonor organ shortage limitshepatocyte differentiationhiPSC
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