ALARM NMR data - HAT inhibitors

Representative [<sup>1</sup>H-<sup>13</sup>C]-HMQC ALARM NMR spectra for reported histone acetyltransferase inhibitors. ALARM NMR is a protein-based NMR counter-screen for flagging potentially promiscuous and/or assay interference compounds in drug and chemical probe discovery.<br><br>Human La antigen (50 uM final concentration) was incubated with reported HAT inhibitors (400 uM final concentration) in 25 mM sodium phosphate, pH 7.0, 10% D<sub>2</sub>O, in the presence or absence of DTT (0 or 25 mM final concentration).<br><br>Data was obtained on a 700 MHz Bruker Avance NMR spectrometer equipped with TCI Cryoprobe. Compressed files (.zip) are in standard Bruker format and were analyzed in TopSpin 3.5.<br><br>Controls: DMSO (vehicle), CPM (thiol-reactive control compound), fluconazole (negative control compound).<br><br>Files are listed according to compound tested (number in accompanying manuscript) and DTT status (DTT, NoDTT).<br>