ADAR hypomorphs display weaker adaptation of their transcriptome to temperature shifts.
(A) 'Volcano plot' showing gene expression differences in 29°C vs. 18°C in WT and ADAR hypomorph flies. Colored dots indicate genes significantly changing between the temperatures (p < 0.05, log2 (fold change) > 1). (B) Fold change levels of differentially expressed genes in 29°C vs. 18°C (selected as described in A) are plotted in a log scale WT against ADAR hypomorph. Orange dots indicate genes in which the fold change ratio between WT and ADAR hypomorph is > 2 or < 0.5 (C) Editing index, fraction of inosines among all expressed adenosines of all detected editing sites, for differentially expressed genes between control and ADAR hypomorph flies (light bars). Brown bars donate for editing index in all expressed genes. Black bars donates for editing index in control set of 60% most expressed genes. The p.values were calculated using bootstrapping exam with 10,000 random sampling from the control set. The editing levels were calculated on wild type CantonS strain. (D) Cumulative distribution plot for all genes differentially expressed between 29°C and 18°C. Kolmogorov-Smirnov test was used to determine the statistical significance of the differences between the curves (p < 0.0001). (E) Fold change levels of differentially expressed genes in 29°C vs. 18°C that are affected by ADAR hypomorph only in 18°C. Plot was generated as described in B. (F) Cumulative distribution plot of genes differentially expressed at 29°C vs. 18°C that are affected by ADAR hypomorph only in 18°C. Kolmogorov-Smirnov test was used to determine the statistical significance of the differences between the curves (p < 0.001).
