ACT induces astrocyte damage.

<p>(A) Astrocyte cells were co-stained with Hoechst 33342 and propidium iodide (PI). Cells were treated with NH<sub>4</sub>Cl (5 mM) and ACT (0.5 mg/ml) for 48 h. Final observation was conducted by fluorescence microscopy. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst-stained cells. Cells stained by PI represent dead cells, whereas Hoechst 33342 staining reveals all nuclei. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p>