ACT increases AQP4 expression in astrocytes.

<p>Time course of the expression of AQP4 mRNA and protein in astrocytes at 6, 12, 24, and 48 h after treatment with NH<sub>4</sub>Cl and ACT, respectively. (A, B) The expression of AQP4 mRNA was analyzed by quantitative real-time RT-PCR and compared to the respective untreated controls. The expression, standardized by the level of GAPDH, is shown as folds of expression. (C, D) The AQP4 protein level was analyzed by Western blotting with an AQP4-specific monoclonal antibody. Band densities were analyzed using the Image Lab 5.1 software program (Bio-Rad) and are expressed as a percentage of the untreated control. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control.</p>