10.1371/journal.pone.0223716
Ahmed El Bakkali
Ahmed El
Bakkali
Laila Essalouh
Laila
Essalouh
Christine Tollon
Christine
Tollon
Ronan Rivallan
Ronan
Rivallan
Pierre Mournet
Pierre
Mournet
Abdelmajid Moukhli
Abdelmajid
Moukhli
Hayat Zaher
Hayat
Zaher
Abderrahmane Mekkaoui
Abderrahmane
Mekkaoui
Amal Hadidou
Amal
Hadidou
Lhassane Sikaoui
Lhassane
Sikaoui
Bouchaib Khadari
Bouchaib
Khadari
Characterization of Worldwide Olive Germplasm Banks of Marrakech (Morocco) and Córdoba (Spain): Towards management and use of olive germplasm in breeding programs
Public Library of Science
2019
germplasm collections
120 authenticated cultivars
fruit crop
192 mislabeling errors
20 microsatellite markers
C órdoba
74 cultivars
WOGB
79 synonyms
diversity index
core collections
cultivar variability
collection establishment conditions
endocarp traits
150 sample sizes
Worldwide Olive Germplasm Bank
11 endocarp
39 homonyms
research teams
programs Olive
Mediterranean Basin
varietal composition
diversity level
allelic richness
SSR markers
WOGBM
WOGBC
phenotyping accessions
228 genotypes
SSR profiles
conduct association mapping studies
Worldwide Olive Germplasm Banks
2019-10-17 17:52:23
Dataset
https://plos.figshare.com/articles/dataset/Characterization_of_Worldwide_Olive_Germplasm_Banks_of_Marrakech_Morocco_and_C_rdoba_Spain_Towards_management_and_use_of_olive_germplasm_in_breeding_programs/9997784
<div><p>Olive (<i>Olea europaea</i> L.) is a major fruit crop in the Mediterranean Basin. <i>Ex-situ</i> olive management is essential to ensure optimal use of genetic resources in breeding programs. The Worldwide Olive Germplasm Bank of Córdoba (WOGBC), Spain, and Marrakech (WOGBM), Morocco, are currently the largest existing olive germplasm collections. Characterization, identification, comparison and authentication of all accessions in both collections could thus provide useful information for managing olive germplasm for its preservation, exchange within the scientific community and use in breeding programs. Here we applied 20 microsatellite markers (SSR) and 11 endocarp morphological traits to discriminate and authenticate 1091 olive accessions belonging to WOGBM and WOGBC (554 and 537, respectively). Of all the analyzed accessions, 672 distinct SSR profiles considered as unique genotypes were identified, but only 130 were present in both collections. Combining SSR markers and endocarp traits led to the identification of 535 cultivars (126 in common) and 120 authenticated cultivars. No significant differences were observed between collections regarding the allelic richness and diversity index. We concluded that the genetic diversity level was stable despite marked contrasts in varietal composition between collections, which could be explained by their different collection establishment conditions. This highlights the extent of cultivar variability within WOGBs. Moreover, we detected 192 mislabeling errors, 72 of which were found in WOGBM. A total of 228 genotypes as molecular variants of 74 cultivars, 79 synonyms and 39 homonyms as new cases were identified. Both collections were combined to define the nested core collections of 55, 121 and 150 sample sizes proposed for further studies. This study was a preliminary step towards managing and mining the genetic diversity in both collections while developing collaborations between olive research teams to conduct association mapping studies by exchanging and phenotyping accessions in contrasted environmental sites.</p></div>