Thuy Duong, Nguyen Morris, Glenn E. Lam, Le Thanh Zhang, Qiuping A. Sewry, Caroline M. Shanahan, Catherine Holt, Ian Evidence at the protein level for nesprin-2-epsilon-1 in Ntera-2 and ESC cells and for nesprin-2-epsilon-2 in heart and brain. <p>Western blot for nesprin-2 in human tissues and cell lines using antibody against the C-terminal region of nesprin-2 (MANNES2A 11A3). Bands of approximate size of nesprin-2-epsilon-1 (122 kD) were detected in Ntera-2 and ESC and bands the size of nesprin-2-epsilon-2 (103 kD) were detected in brain and heart. Nesprin-2-alpha-1 was observed in skeletal muscle, but epsilon isoforms were not detected. Absence of mRNA (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094380#pone.0094380.s001" target="_blank">Table S4 in File S1</a>), indicates that putative nesprin-2-gamma (377 kD) and nesprin-2-alpha-2 (47 kD) bands on the skeletal muscle western blot, are likely to be degradation products.</p> cell biology;Cellular structures and organelles;Cell membranes;Membrane proteins;Transmembrane proteins;Molecular cell biology;Biochemistry;dna;DNA amplification;proteins;Structural proteins;rna;RNA synthesis;Nucleic acids;immunology;immunochemistry;genetics;gene expression;Gene regulation;Genetic dominance;Autosomal dominant traits;Human genetics;Molecular genetics;cardiology;Cardiovascular diseases;cardiomyopathies;neurology;Muscular dystrophies;nesprin-2-epsilon-1;ntera-2;esc;cells;nesprin-2-epsilon-2 2014-04-09
    https://plos.figshare.com/articles/figure/_Evidence_at_the_protein_level_for_nesprin_2_epsilon_1_in_Ntera_2_and_ESC_cells_and_for_nesprin_2_epsilon_2_in_heart_and_brain_/993473
10.1371/journal.pone.0094380.g005