Evidence at the protein level for nesprin-2-epsilon-1 in Ntera-2 and ESC cells and for nesprin-2-epsilon-2 in heart and brain. Nguyen Thuy Duong Glenn E. Morris Le Thanh Lam Qiuping Zhang Caroline A. Sewry Catherine M. Shanahan Ian Holt 10.1371/journal.pone.0094380.g005 https://plos.figshare.com/articles/figure/_Evidence_at_the_protein_level_for_nesprin_2_epsilon_1_in_Ntera_2_and_ESC_cells_and_for_nesprin_2_epsilon_2_in_heart_and_brain_/993473 <p>Western blot for nesprin-2 in human tissues and cell lines using antibody against the C-terminal region of nesprin-2 (MANNES2A 11A3). Bands of approximate size of nesprin-2-epsilon-1 (122 kD) were detected in Ntera-2 and ESC and bands the size of nesprin-2-epsilon-2 (103 kD) were detected in brain and heart. Nesprin-2-alpha-1 was observed in skeletal muscle, but epsilon isoforms were not detected. Absence of mRNA (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094380#pone.0094380.s001" target="_blank">Table S4 in File S1</a>), indicates that putative nesprin-2-gamma (377 kD) and nesprin-2-alpha-2 (47 kD) bands on the skeletal muscle western blot, are likely to be degradation products.</p> 2014-04-09 03:41:15 cell biology Cellular structures and organelles Cell membranes Membrane proteins Transmembrane proteins Molecular cell biology Biochemistry dna DNA amplification proteins Structural proteins rna RNA synthesis Nucleic acids immunology immunochemistry genetics gene expression Gene regulation Genetic dominance Autosomal dominant traits Human genetics Molecular genetics cardiology Cardiovascular diseases cardiomyopathies neurology Muscular dystrophies nesprin-2-epsilon-1 ntera-2 esc cells nesprin-2-epsilon-2