10.6084/m9.figshare.9750602.v1 Paul Müller Paul Müller Mirjam Schürmann Mirjam Schürmann Salvatore Girardo Salvatore Girardo Gheorghe Cojoc Gheorghe Cojoc Jochen Guck Jochen Guck Quantitative phase microscopy images of spherical droplets, beads, and cells figshare 2019 Digital holography Phase shift Mie theory Propagation methods Index measurements Cell analysis Biophysics Human Biophysics 2019-08-30 05:14:41 Dataset https://figshare.com/articles/dataset/Quantitative_phase_microscopy_images_of_spherical_droplets_beads_and_cells/9750602 Example data sets for quantitative phase imaging.<br><br>These data were used by Müller et al. [1] to demonstrate the<br>accurate retrieval of size and refractive index for spherical<br>objects by means of a two-dimensional phase data fitting<br>algorithm.<br><br>Abbreviations:<br><br> - DHM: digital holographic microscopy<br> - FUS: fused in sarcoma (RNA-binding protein)<br> - HL60: name of a human leukemia cell line<br> - QLSI: quadri-wave lateral shearing interferometry<br> - PAA: Polyacrylamide (hydrogel)<br> - PBS: phosphate buffered saline (buffer medium)<br><br>The data are related to [1] according to the following scheme:<br><br>Figure 4:<br><br> a-c) SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07<br> Silicone oil droplet measured in PBS using QLSI<br> d-f) SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5<br> FUS protein droplet measured in egg extract using QLSI<br> (included in fig. 5a)<br> g-j) SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23<br> PAA hydrogel bead (referred to as "type A" in [1]) measured<br> in PBS using QLSI (included in fig. 5b)<br> k-m) DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11<br> PAA hydrogel bead (referred to as "type B" in [1]) measured<br> in PBS using DHM<br> n-q) QLSI_2015-11-09_16-03-52_HL60<br> HL60 cells measured in PBS using QLSI (included in fig. 5c)<br> r-s) DHM_2015-11-09_16-48-45_HL60<br> HL60 cells measured in PBS using DHM (included in fig. 5d)<br><br>Figure 5:<br><br> a) QLSI_2016-04-29_FUS-droplet-780-5<br> Time lapse measurement of a FUS protein droplet measured in<br> egg extract using QLSI<br> b) QLSI_2016-07-05_PAA-beads-S6-A<br> PAA hydrogel beads (referred to as "type A" in [1]) measured<br> in PBS using QLSI<br> c) QLSI_2015-11-09_HL60<br> HL60 cells measured in PBS using QLSI<br> d) DHM_2015-11-09_HL60<br> HL60 cells measured in PBS using DHM<br><br>The data are stored in different file formats:<br><br> - .tif: digital holography data or (!) SID4Bio (Phasics S.A.) data<br> - .h5: HDF5-based qpimage [2] file format<br> - .zip: each ZIP file contains several .tif files<br> - .cfg: meta data and analysis recipe for each data set,<br> stored as a DryMass [3] profile<br><br>The phase data can be extracted with DryMass [3] and qpformat [4],<br>e.g. using the command-line interface of DryMass:<br><br> dm_analyze_sphere --profile DHM_2015-11-09_HL60.cfg DHM_2015-11-09_HL60.zip<br><br>For more information and a detailed analysis, please refer to [1].<br><br><br>License: CC0 (https://creativecommons.org/publicdomain/zero/1.0/)<br><br><br>SHA256 sums:<br><br>cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_16-48-45_HL60.cfg<br>04eaf59da61934486385f42600aec0a2ffb3f71c93c2b6b6f247ac237088eefb DHM_2015-11-09_16-48-45_HL60.tif<br>cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_HL60.cfg<br>64e7b0e6fab4d947b82333ea2edd20b41ce51c813c97ae1070da90e58de35b02 DHM_2015-11-09_HL60.zip<br>685c886d44b9359dca1b2d2bd504f2b1d5f149c05da5a1263ee802c02fb41e66 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.cfg<br>75608e106ecf41a22d707c966faf850f1414e9f0012ce69104658680b4b26876 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.tif<br>138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_16-03-52_HL60.cfg<br>73dd8612b713b62227f52e68619f2e5794adabd29c09622b985fdcc842eb68ff QLSI_2015-11-09_16-03-52_HL60.h5<br>138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_HL60.cfg<br>9f3af6ccdd0a71e53d2907ee8f7b6e478c56da99d3dac9a443aceaa4b9f1658e QLSI_2015-11-09_HL60.h5<br>1a95d8bfcee3e4b442afccd88d0fa89ed9013fe0c96bf5a337ede481eef1f99d QLSI_2016-04-29_FUS-droplet-780-5.cfg<br>f9a407ca31f3aaa106aa7b5db74ab74b9244e9624212e312ce3c82afa60ef714 QLSI_2016-04-29_FUS-droplet-780-5.zip<br>3dc230d213d788d194b053c04ed019104207aa631eac27924215232dce0b0773 QLSI_2016-07-05_PAA-beads-S6-A.cfg<br>7fa1becd016dcd3bf28c8c4c693e15110d239dca803414eed9eb0a62bc5a9daf QLSI_2016-07-05_PAA-beads-S6-A.zip<br>ea8143e609217dc54e2ded3185ff4b88605c995376a4b86cb297a44aa4ae7baf SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.cfg<br>5819ec142b58fd7ef9df614a4ac63c78efdaf9ae9e8f02b5ce2f6215fd9251d1 SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.tif<br>0b478d422d6510aa4cf8b5d082128c595cac4a4d3b1ce4c53c8b496d410299cf SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.cfg<br>ab9503b33562968333423b5a541ee41cac135fa1153a835da3c009f9f5c305ed SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.tif<br>3bb6ed2275f61d03628e5dbea861b7fe87870cd17cd998679cd14da30aaab274 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.cfg<br>e2b3d79bf3b7904a9184b79746dbeff696f716e6789541ff6835672514a25a93 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.tif<br><br><br>References:<br><br>[1] https://dx.doi.org/10.1364/OE.26.010729<br>[2] https://qpimage.readthedocs.io<br>[3] https://drymass.readthedocs.io<br>[4] https://qpformat.readthedocs.io<br><br>