10.1371/journal.pone.0092172.g003
Shiping Jiao
Shiping
Jiao
Minqing Wu
Minqing
Wu
Feng Ye
Feng
Ye
Hailin Tang
Hailin
Tang
Xinhua Xie
Xinhua
Xie
Xiaoming Xie
Xiaoming
Xie
BikDDA eliminated TNBC cells more powerfully than BikDD.
Public Library of Science
2014
Biochemistry
cell biology
Cell processes
Cell death
Molecular cell biology
molecular biology
Molecular biology techniques
Gene therapy
Clinical genetics
epidemiology
Cancer epidemiology
Molecular epidemiology
oncology
Cancers and neoplasms
Breast tumors
breast cancer
Cancer treatment
pharmacology
Drug research and development
drug discovery
eliminated
tnbc
cells
powerfully
2014-03-17 03:08:22
Figure
https://plos.figshare.com/articles/figure/_BikDDA_eliminated_TNBC_cells_more_powerfully_than_BikDD_/964067
<p>A. S124A and previous mutant sites T33D and S35D didn’t locate in the pro-apoptotic region of Bik. B. MDA-MB-231 cells were transfected for 28h with the same amount of pUK21, pUK21-BikDD or pUK21-BikDDA. Apoptotic cells were monitored by Annexin V/PI staining and flow-cytometry analysis. The right-lower or right upper quadrant of each plot showed early apoptotic or late apoptotic cells. C. Histogram directly showed the enhanced apoptosis-inducing activity of BikDDA. D. The cytotoxicity of BikDD and BikDDA were analyzed by Cell counting kit and MTT assay (setting at 100% in vector group). E. Western blotting was performed to confirm the prolonged half-life of BikDDA in 231 cells. F. MEK1/2 inhibitor UO126 and selective ERK1/2 inhibitor FR180204 increased the stability of BikDD in 231 cells.</p>