10.1371/journal.pone.0092172.g003 Shiping Jiao Shiping Jiao Minqing Wu Minqing Wu Feng Ye Feng Ye Hailin Tang Hailin Tang Xinhua Xie Xinhua Xie Xiaoming Xie Xiaoming Xie BikDDA eliminated TNBC cells more powerfully than BikDD. Public Library of Science 2014 Biochemistry cell biology Cell processes Cell death Molecular cell biology molecular biology Molecular biology techniques Gene therapy Clinical genetics epidemiology Cancer epidemiology Molecular epidemiology oncology Cancers and neoplasms Breast tumors breast cancer Cancer treatment pharmacology Drug research and development drug discovery eliminated tnbc cells powerfully 2014-03-17 03:08:22 Figure https://plos.figshare.com/articles/figure/_BikDDA_eliminated_TNBC_cells_more_powerfully_than_BikDD_/964067 <p>A. S124A and previous mutant sites T33D and S35D didn’t locate in the pro-apoptotic region of Bik. B. MDA-MB-231 cells were transfected for 28h with the same amount of pUK21, pUK21-BikDD or pUK21-BikDDA. Apoptotic cells were monitored by Annexin V/PI staining and flow-cytometry analysis. The right-lower or right upper quadrant of each plot showed early apoptotic or late apoptotic cells. C. Histogram directly showed the enhanced apoptosis-inducing activity of BikDDA. D. The cytotoxicity of BikDD and BikDDA were analyzed by Cell counting kit and MTT assay (setting at 100% in vector group). E. Western blotting was performed to confirm the prolonged half-life of BikDDA in 231 cells. F. MEK1/2 inhibitor UO126 and selective ERK1/2 inhibitor FR180204 increased the stability of BikDD in 231 cells.</p>