3D tumor spheroid culture alters basal EGFR and cMET phosphorylation and response to ligand stimulation. Jason E. Ekert Kjell Johnson Brandy Strake Jose Pardinas Stephen Jarantow Robert Perkinson David C. Colter 10.1371/journal.pone.0092248.g003 https://plos.figshare.com/articles/figure/_3D_tumor_spheroid_culture_alters_basal_EGFR_and_cMET_phosphorylation_and_response_to_ligand_stimulation_/964066 <p>Day four 2D monolayer cultures and 3D spheroid from eight lung tumor cell lines were stimulated with or without 100/ml of EGF or HGF for 15 minutes. Phosphorylation for EGFR and cMET was determined by MSD assay. N is equal to 4 replicates per condition. T-test comparing basal phosphorylation in 2D verses 3D cultures. (* indicates p<0.05 and ** indicates p<0.005).</p> 2014-03-17 03:11:23 biotechnology immunology Clinical immunology Antibody therapy physiology Endocrine physiology Growth factors Epidermal growth factor oncology Cancers and neoplasms carcinomas adenocarcinomas Adenocarcinoma of the lung Lung and intrathoracic tumors Non-small cell lung cancer Cancer treatment pharmacology Drug research and development drug discovery spheroid alters basal egfr cmet phosphorylation ligand