3D tumor spheroid culture alters basal EGFR and cMET phosphorylation and response to ligand stimulation.
Jason E. Ekert
Kjell Johnson
Brandy Strake
Jose Pardinas
Stephen Jarantow
Robert Perkinson
David C. Colter
10.1371/journal.pone.0092248.g003
https://plos.figshare.com/articles/figure/_3D_tumor_spheroid_culture_alters_basal_EGFR_and_cMET_phosphorylation_and_response_to_ligand_stimulation_/964066
<p>Day four 2D monolayer cultures and 3D spheroid from eight lung tumor cell lines were stimulated with or without 100/ml of EGF or HGF for 15 minutes. Phosphorylation for EGFR and cMET was determined by MSD assay. N is equal to 4 replicates per condition. T-test comparing basal phosphorylation in 2D verses 3D cultures. (* indicates p<0.05 and ** indicates p<0.005).</p>
2014-03-17 03:11:23
biotechnology
immunology
Clinical immunology
Antibody therapy
physiology
Endocrine physiology
Growth factors
Epidermal growth factor
oncology
Cancers and neoplasms
carcinomas
adenocarcinomas
Adenocarcinoma of the lung
Lung and intrathoracic tumors
Non-small cell lung cancer
Cancer treatment
pharmacology
Drug research and development
drug discovery
spheroid
alters
basal
egfr
cmet
phosphorylation
ligand