Cell characterization after long term growth on Synthemax II microcarriers in xeno-free medium.
Martial Hervy
Jennifer L. Weber
Marylene Pecheul
Paula Dolley-Sonneville
David Henry
Yue Zhou
Zara Melkoumian
10.1371/journal.pone.0092120.g005
https://plos.figshare.com/articles/figure/_Cell_characterization_after_long_term_growth_on_Synthemax_II_microcarriers_in_xeno_free_medium_/964055
<p>(A) Fluorescent image of hMSCs on Synthemax II microcarriers after calcein AM staining. (B) Phase image of hMSCs after migration off Synthemax II microcarriers to a 6-well plate. (C) hMSC karyotype analysis after 7 passages on Synthemax II microcarriers in stemgro hMSC medium. (D) After 5 passages on Synthemax II microcarriers, hMSCs were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages and were stained with oil red O, alizarin red, and alcian blue, respectively. Representative images of stained cells from both media are shown.</p>
2014-03-17 03:10:01
biotechnology
biomaterials
cell biology
Cell processes
Cell growth
Cellular types
Animal cells
stem cells
mesenchymal stem cells
Stem cell lines
Molecular cell biology
developmental biology
Materials Science
characterization
synthemax
ii
microcarriers
xeno-free