Cell characterization after long term growth on Synthemax II microcarriers in xeno-free medium. Martial Hervy Jennifer L. Weber Marylene Pecheul Paula Dolley-Sonneville David Henry Yue Zhou Zara Melkoumian 10.1371/journal.pone.0092120.g005 https://plos.figshare.com/articles/figure/_Cell_characterization_after_long_term_growth_on_Synthemax_II_microcarriers_in_xeno_free_medium_/964055 <p>(A) Fluorescent image of hMSCs on Synthemax II microcarriers after calcein AM staining. (B) Phase image of hMSCs after migration off Synthemax II microcarriers to a 6-well plate. (C) hMSC karyotype analysis after 7 passages on Synthemax II microcarriers in stemgro hMSC medium. (D) After 5 passages on Synthemax II microcarriers, hMSCs were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages and were stained with oil red O, alizarin red, and alcian blue, respectively. Representative images of stained cells from both media are shown.</p> 2014-03-17 03:10:01 biotechnology biomaterials cell biology Cell processes Cell growth Cellular types Animal cells stem cells mesenchymal stem cells Stem cell lines Molecular cell biology developmental biology Materials Science characterization synthemax ii microcarriers xeno-free