%0 Figure %A Chernobrovkin, Alexey L. %A Zubarev, Roman A. %D 2014 %T The suggested three-step workflow of shotgun proteomics analysis that includes contamination/infection detection. %U https://plos.figshare.com/articles/figure/_The_suggested_three_step_workflow_of_shotgun_proteomics_analysis_that_includes_contamination_infection_detection_/958292 %R 10.1371/journal.pone.0091433.g001 %2 https://ndownloader.figshare.com/files/1415482 %K Biochemistry %K proteins %K Protein classes %K biotechnology %K microbiology %K Virology %K Viral classification %K Viral enzymes %K Model organisms %K proteomics %K Protein abundance %K Spectrometric identification of proteins %K Infectious diseases %K Viral diseases %K suggested %K three-step %K workflow %K shotgun %K includes %X

(1) conventional matching the experimental MS/MS spectra against the protein database of the host organism (human); (2) Unmatched MS/MS spectra and insignificant matches in terms of FDR are matched against viral protein database. (3) The presence of significant hits at the second step points to the potential infection/contamination of the sample. Label-free quantitative analysis of identified proteins (both host and contaminant) provides an estimation of the contamination level and thus severity of the problem.

%I PLOS ONE