TY - DATA T1 - Elevated pCO2 causes developmental delay in early larval Pacific oysters, Crassostrea gigas PY - 2012/09/12 AU - Emma Timmins-Schiffman AU - Steven Roberts AU - Michael O'Donnell AU - Carolyn Friedman UR - https://figshare.com/articles/journal_contribution/Elevated_pCO2_causes_developmental_delay_in_early_larval_Pacific_oysters_Crassostrea_gigas/95828 DO - 10.6084/m9.figshare.95828.v1 L4 - https://ndownloader.figshare.com/files/98368 KW - acidification KW - larvae KW - oyster KW - Marine Biology KW - Ecology N2 - **This is a draft version of the following article: Timmins-Schiffman et al. (2012) Elevated pCO2 causes developmental delay in early larval Pacific oysters, Crassostrea gigas. Marine Biology, doi:10.1007/s00227-012-2055-x, which has been published in final form at the link provided below.** Increasing atmospheric CO2 equilibrates with surface seawater, elevating the concentration of aqueous hydrogen ions. This process, ocean acidification, is a future and contemporary concern for aquatic organisms, causing failures in Pacific oyster (Crassostrea gigas) aquaculture.  This experiment determines the effect of elevated pCO2 on the early development of C. gigas larvae from a wild Pacific Northwest population.  Adults were collected from Friday Harbor, Washington, USA (48°31.7’ N, 12°1.1’ W) and spawned in July 2011. Larvae were exposed to Ambient (400 µatm CO2), MidCO2 (700 µatm), or HighCO2 (1000 µatm).  After 24 hours, a greater proportion of larvae in the HighCO2 treatment were calcified as compared to Ambient. This unexpected observation is attributed to increased metabolic rate coupled with sufficient energy resources.  Oyster larvae raised at HighCO2 showed evidence of a developmental delay by 3 days post-fertilization, which resulted in smaller larvae that were less calcified.  ER -