10.1371/journal.pone.0090386.g009
Hans Heid
Hans
Heid
Steffen Rickelt
Steffen
Rickelt
Ralf Zimbelmann
Ralf
Zimbelmann
Stefanie Winter
Stefanie
Winter
Heiderose Schumacher
Heiderose
Schumacher
Yvette Dörflinger
Yvette
Dörflinger
Caecilia Kuhn
Caecilia
Kuhn
Werner W. Franke
Werner
W. Franke
Electron micrographs showing the association of vimentin filaments with the forming of LDs after brief AIM stimulation of human preadipocytes.
Public Library of Science
2014
Biochemistry
lipids
Fatty acids
Lipid aggregates
Lipid metabolism
Lipid structure
metabolism
Metabolic pathways
proteins
Cytoskeletal proteins
lipoproteins
Protein interactions
Structural proteins
immunochemistry
immunology
Immunologic techniques
immunofluorescence
micrographs
vimentin
filaments
lds
stimulation
2014-02-28 04:20:13
Figure
https://plos.figshare.com/articles/figure/_Electron_micrographs_showing_the_association_of_vimentin_filaments_with_the_forming_of_LDs_after_brief_AIM_stimulation_of_human_preadipocytes_/949291
<p>(a) Grazing section of two very small LDs (approx. 200 nm in diameter) surrounded by numerous vimentin IF bundles of the “wide-spacing type” with approximately 30–50 nm spacing distance (cp.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090386#pone.0090386-Franke1" target="_blank">[15]</a>) and with interconnection of LDs by filaments (arrows). (b) Similar small LDs associated with filaments as seen in (a). (c) Survey electron micrograph showing ordered arrays of vimentin bundles at the surface of LDs. Some LDs can be seen interconnected by IFs (arrows). First evidences of specific assembly of smooth ER tubules forming cisternae sheaths and layers can be noticed (brown-colored system of tubules). Note, at this stage of adipocyte differentiation mitochondria (M) are not directly associated to these forming small LDs. Bars: 0.50 µm.</p>