10.1371/journal.pone.0090386.g009 Hans Heid Hans Heid Steffen Rickelt Steffen Rickelt Ralf Zimbelmann Ralf Zimbelmann Stefanie Winter Stefanie Winter Heiderose Schumacher Heiderose Schumacher Yvette Dörflinger Yvette Dörflinger Caecilia Kuhn Caecilia Kuhn Werner W. Franke Werner W. Franke Electron micrographs showing the association of vimentin filaments with the forming of LDs after brief AIM stimulation of human preadipocytes. Public Library of Science 2014 Biochemistry lipids Fatty acids Lipid aggregates Lipid metabolism Lipid structure metabolism Metabolic pathways proteins Cytoskeletal proteins lipoproteins Protein interactions Structural proteins immunochemistry immunology Immunologic techniques immunofluorescence micrographs vimentin filaments lds stimulation 2014-02-28 04:20:13 Figure https://plos.figshare.com/articles/figure/_Electron_micrographs_showing_the_association_of_vimentin_filaments_with_the_forming_of_LDs_after_brief_AIM_stimulation_of_human_preadipocytes_/949291 <p>(a) Grazing section of two very small LDs (approx. 200 nm in diameter) surrounded by numerous vimentin IF bundles of the “wide-spacing type” with approximately 30–50 nm spacing distance (cp.<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090386#pone.0090386-Franke1" target="_blank">[15]</a>) and with interconnection of LDs by filaments (arrows). (b) Similar small LDs associated with filaments as seen in (a). (c) Survey electron micrograph showing ordered arrays of vimentin bundles at the surface of LDs. Some LDs can be seen interconnected by IFs (arrows). First evidences of specific assembly of smooth ER tubules forming cisternae sheaths and layers can be noticed (brown-colored system of tubules). Note, at this stage of adipocyte differentiation mitochondria (M) are not directly associated to these forming small LDs. Bars: 0.50 µm.</p>