TY - DATA T1 - mTOR controls GRP78 synthesis in human cholangiocarcinoma cells. PY - 2014/02/28 AU - Chunhong Feng AU - Kai He AU - Chunyan Zhang AU - Song Su AU - Bo Li AU - Yuxiao Li AU - Chun-Yan Duan AU - Shaokun Chen AU - Run Chen AU - Youping Liu AU - Hong Li AU - Mei Wei AU - Xianming Xia AU - Rongyang Dai UR - https://plos.figshare.com/articles/figure/_mTOR_controls_GRP78_synthesis_in_human_cholangiocarcinoma_cells_/949289 DO - 10.1371/journal.pone.0090388.g006 L4 - https://ndownloader.figshare.com/files/1404305 KW - biophysics KW - Protein folding KW - Molecular cell biology KW - Signal transduction KW - Signaling cascades KW - c-Jun N-terminal kinase signaling cascade KW - Signaling in selected disciplines KW - Oncogenic signaling KW - Cellular stress responses KW - Gastroenterology and hepatology KW - Gastrointestinal cancers KW - oncology KW - Cancers and neoplasms KW - Gastrointestinal tumors KW - controls KW - grp78 KW - synthesis KW - cholangiocarcinoma N2 - (A) After treated with rapamycin (Rap, 20 nM) for indicated time periods, GRP78 in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (B) After transfected with simTOR for 60 h, GRP78 in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without rapamycin (Rap, 20 nM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells. (D) After treated with tunicamycin (Tun, 2.0 µg/ml) for 24 h with or without rapamycin (Rap, 20 nM) preincubation for 1 h, GRP78 in HepG2 cells was analyzed using western blot. ER -