mTOR controls GRP78 synthesis in human cholangiocarcinoma cells. Chunhong Feng Kai He Chunyan Zhang Song Su Bo Li Yuxiao Li Chun-Yan Duan Shaokun Chen Run Chen Youping Liu Hong Li Mei Wei Xianming Xia Rongyang Dai 10.1371/journal.pone.0090388.g006 https://plos.figshare.com/articles/figure/_mTOR_controls_GRP78_synthesis_in_human_cholangiocarcinoma_cells_/949289 <p>(A) After treated with rapamycin (Rap, 20 nM) for indicated time periods, GRP78 in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (B) After transfected with simTOR for 60 h, GRP78 in QBC939, RBE and HCCC-9810 cells was analyzed using western blot. (C) After treated with salubrinal (Sal, 25 µM) for 30 h with or without rapamycin (Rap, 20 nM) preincubation for 1 h, GRP78 was analyzed using western blot in HepG2 cells. (D) After treated with tunicamycin (Tun, 2.0 µg/ml) for 24 h with or without rapamycin (Rap, 20 nM) preincubation for 1 h, GRP78 in HepG2 cells was analyzed using western blot.</p> 2014-02-28 04:18:45 biophysics Protein folding Molecular cell biology Signal transduction Signaling cascades c-Jun N-terminal kinase signaling cascade Signaling in selected disciplines Oncogenic signaling Cellular stress responses Gastroenterology and hepatology Gastrointestinal cancers oncology Cancers and neoplasms Gastrointestinal tumors controls grp78 synthesis cholangiocarcinoma