10.1371/journal.pone.0089396.g005 Jin-Bon Hong Jin-Bon Hong Fu-Ju Chou Fu-Ju Chou Amy T. Ku Amy T. Ku Hsiang-Hsuan Fan Hsiang-Hsuan Fan Tung-Lung Lee Tung-Lung Lee Yung-Hsin Huang Yung-Hsin Huang Tsung-Lin Yang Tsung-Lin Yang I-Chang Su I-Chang Su I-Shing Yu I-Shing Yu Shu-Wha Lin Shu-Wha Lin Chung-Liang Chien Chung-Liang Chien Hong-Nerng Ho Hong-Nerng Ho You-Tzung Chen You-Tzung Chen Stability analysis of PBase protein, facilitated by flow cytometry. Public Library of Science 2014 biotechnology Genetic engineering genetics Human genetics Gene therapy genomics Functional genomics Molecular cell biology Cellular structures nucleolus Cellular types stem cells Embryonic stem cells transposons DNA transposons pbase facilitated 2014-02-24 03:36:18 Figure https://plos.figshare.com/articles/figure/_Stability_analysis_of_PBase_protein_facilitated_by_flow_cytometry_/943406 <p>Hela cells were transfected with <i>pTriEx-mPB-2A-eGFP</i> and <i>pTriEx-NP-mPB-2A-eGFP</i> plasmids. (A) APC fluorescence via anti-His tag antibody staining indicated the presence of mPB or NP-mPB. Because the eGFP moiety was cotranslated with PBase variants, the GFP fluorescence intensity was used as a reference for the mPB and NP-mPB translation levels of successfully transfected cells. (B and C) Y-axis represents accumulated cell counts. Although the NP-mPB group had a much smaller percentage of cells that were APC+ (4.65%) than the mPB group (18.2%), the percentages of GFP+ cells were similar (26.7% vs. 32.7%). (D) The APC fluorescence intensity in GFP+ populations represented the protein stability. The ratio of mean APC fluorescence (a.u.) of NP-mPB to mPB was 302 to 671.</p>