10.1371/journal.pone.0089396.g005
Jin-Bon Hong
Jin-Bon
Hong
Fu-Ju Chou
Fu-Ju
Chou
Amy T. Ku
Amy
T. Ku
Hsiang-Hsuan Fan
Hsiang-Hsuan
Fan
Tung-Lung Lee
Tung-Lung
Lee
Yung-Hsin Huang
Yung-Hsin
Huang
Tsung-Lin Yang
Tsung-Lin
Yang
I-Chang Su
I-Chang
Su
I-Shing Yu
I-Shing
Yu
Shu-Wha Lin
Shu-Wha
Lin
Chung-Liang Chien
Chung-Liang
Chien
Hong-Nerng Ho
Hong-Nerng
Ho
You-Tzung Chen
You-Tzung
Chen
Stability analysis of PBase protein, facilitated by flow cytometry.
Public Library of Science
2014
biotechnology
Genetic engineering
genetics
Human genetics
Gene therapy
genomics
Functional genomics
Molecular cell biology
Cellular structures
nucleolus
Cellular types
stem cells
Embryonic stem cells
transposons
DNA transposons
pbase
facilitated
2014-02-24 03:36:18
Figure
https://plos.figshare.com/articles/figure/_Stability_analysis_of_PBase_protein_facilitated_by_flow_cytometry_/943406
<p>Hela cells were transfected with <i>pTriEx-mPB-2A-eGFP</i> and <i>pTriEx-NP-mPB-2A-eGFP</i> plasmids. (A) APC fluorescence via anti-His tag antibody staining indicated the presence of mPB or NP-mPB. Because the eGFP moiety was cotranslated with PBase variants, the GFP fluorescence intensity was used as a reference for the mPB and NP-mPB translation levels of successfully transfected cells. (B and C) Y-axis represents accumulated cell counts. Although the NP-mPB group had a much smaller percentage of cells that were APC+ (4.65%) than the mPB group (18.2%), the percentages of GFP+ cells were similar (26.7% vs. 32.7%). (D) The APC fluorescence intensity in GFP+ populations represented the protein stability. The ratio of mean APC fluorescence (a.u.) of NP-mPB to mPB was 302 to 671.</p>