The mPB and NP-mPB protein expression profiles in HEK293T cells transfected with <i>pTriEx-mPB</i> and <i>pTriEx-NP-mPB</i> plasmids. Jin-Bon Hong Fu-Ju Chou Amy T. Ku Hsiang-Hsuan Fan Tung-Lung Lee Yung-Hsin Huang Tsung-Lin Yang I-Chang Su I-Shing Yu Shu-Wha Lin Chung-Liang Chien Hong-Nerng Ho You-Tzung Chen 10.1371/journal.pone.0089396.g004 https://plos.figshare.com/articles/figure/_The_mPB_and_NP_mPB_protein_expression_profiles_in_HEK293T_cells_transfected_with_pTriEx_mPB_and_pTriEx_NP_mPB_plasmids_/943404 <p>(A) At 48 h after transfection, the transposase protein level in the <i>pTriEx-mPB</i> group was higher than that in the <i>pTriEx-NP-mPB</i> group. <i>UGm</i> plasmid was cotransfected as a control for transfection efficiency (anti-GFP). <i>pTriEX-HTNC</i> plasmid was transfected as a positive control for anti-His tag antibody (Cre). (B) Western blot analysis of nucleo-cytoplasmic separated lysates revealed that the mPB protein was distributed preferentially in the nucleus and the cytoplasm, whereas almost all NP-mPB protein was localized in the nucleus.</p> 2014-02-24 03:36:18 biotechnology Genetic engineering genetics Human genetics Gene therapy genomics Functional genomics Molecular cell biology Cellular structures nucleolus Cellular types stem cells Embryonic stem cells transposons DNA transposons mpb np-mpb profiles hek293t cells transfected