The mPB and NP-mPB protein expression profiles in HEK293T cells transfected with <i>pTriEx-mPB</i> and <i>pTriEx-NP-mPB</i> plasmids.
Jin-Bon Hong
Fu-Ju Chou
Amy T. Ku
Hsiang-Hsuan Fan
Tung-Lung Lee
Yung-Hsin Huang
Tsung-Lin Yang
I-Chang Su
I-Shing Yu
Shu-Wha Lin
Chung-Liang Chien
Hong-Nerng Ho
You-Tzung Chen
10.1371/journal.pone.0089396.g004
https://plos.figshare.com/articles/figure/_The_mPB_and_NP_mPB_protein_expression_profiles_in_HEK293T_cells_transfected_with_pTriEx_mPB_and_pTriEx_NP_mPB_plasmids_/943404
<p>(A) At 48 h after transfection, the transposase protein level in the <i>pTriEx-mPB</i> group was higher than that in the <i>pTriEx-NP-mPB</i> group. <i>UGm</i> plasmid was cotransfected as a control for transfection efficiency (anti-GFP). <i>pTriEX-HTNC</i> plasmid was transfected as a positive control for anti-His tag antibody (Cre). (B) Western blot analysis of nucleo-cytoplasmic separated lysates revealed that the mPB protein was distributed preferentially in the nucleus and the cytoplasm, whereas almost all NP-mPB protein was localized in the nucleus.</p>
2014-02-24 03:36:18
biotechnology
Genetic engineering
genetics
Human genetics
Gene therapy
genomics
Functional genomics
Molecular cell biology
Cellular structures
nucleolus
Cellular types
stem cells
Embryonic stem cells
transposons
DNA transposons
mpb
np-mpb
profiles
hek293t
cells
transfected