%0 Figure %A Farkas, Daniela %A Kraskauskas, Donatas %A I. Drake, Jennifer %A A. Alhussaini, Aysar %A Kraskauskiene, Vita %A J. Bogaard, Harm %A D. Cool, Carlyne %A F. Voelkel, Norbert %A Farkas, Laszlo %D 2014 %T Proliferation of c-kit+ cells in the lungs of AMD3100-treated SU5416/chronic hypoxia (SuHx) animals. %U https://plos.figshare.com/articles/figure/_Proliferation_of_c_kit_cells_in_the_lungs_of_AMD3100_treated_SU5416_chronic_hypoxia_SuHx_animals_/943326 %R 10.1371/journal.pone.0089810.g008 %2 https://ndownloader.figshare.com/files/1397027 %K Anatomy and physiology %K cardiovascular system %K Circulatory physiology %K Model organisms %K Animal models %K rat %K Molecular cell biology %K Cellular types %K Endothelial cells %K cardiovascular %K Pulmonary vascular diseases %K Vascular biology %K cells %K lungs %K amd3100-treated %K hypoxia %X

(A) Representative optical sections (confocal microscopy) demonstrate c-kit+ von Willebrand Factor+ (vWF+) proliferating cell nuclear antigen+ (PCNA+) cells (upper row) or c-kit+ α-smooth muscle actin+ (α-SMA+) PCNA+ cells (lower row) in pulmonary arteries of SuHx + vehicle and SuHx + AMD3100 treated animals. The inserts show a triple positive cell (arrow) in more detail. Please note that the bright white stained dots indicate cells with very strong PCNA staining. Nuclear counterstaining: 4',6-diamidino-2-phenylindole (DAPI). Magnification: 630×. Scale bar: 20 µm. (B-C) Quantification of the number of c-kit+, c-kit+ vWF+ and c-kit+ α-SMA+, as well as PCNA+, c-kit+ PCNA+, c-kit+ vWF+ PCNA+ and c-kit+ α-SMA+ PCNA+ cells in and around the pulmonary arteries of SuHx + vehicle and SuHx + AMD3100 animals. n = 3 animals per group. * P<0.05 and ** P<0.01.

%I PLOS ONE