Proliferation and apoptosis in the lungs of AMD3100-treated SU5416/chronic hypoxia (SuHx) animals.
Daniela Farkas
Donatas Kraskauskas
Jennifer I. Drake
Aysar A. Alhussaini
Vita Kraskauskiene
Harm J. Bogaard
Carlyne D. Cool
Norbert F. Voelkel
Laszlo Farkas
10.1371/journal.pone.0089810.g007
https://plos.figshare.com/articles/figure/_Proliferation_and_apoptosis_in_the_lungs_of_AMD3100_treated_SU5416_chronic_hypoxia_SuHx_animals_/943324
<p>(<b>A</b>) Representative Western blot for proliferating cell nuclear antigen (PCNA) and cleaved caspase-3 in the lung tissue protein lysate of naïve control animals (n = 3), SuHx + vehicle (n = 6) and SuHx + AMD3100 treated rats (n = 6). β-actin was used as loading control. (<b>B-C</b>) Densitometric analysis indicates increased PCNA (B) and cleaved caspase-3 (C) protein levels in the lungs of SuHx + vehicle animals vs. controls, and that AMD3100 treatment significantly reduced PCNA and cleaved caspase-3 protein levels in the lungs of SuHx animals. Densitometric values were normalized vs. β-actin and expressed as n-fold of naïve controls. n = 3 animals per group for controls and n = 6 animals per group for SuHx + vehicle and SuHx + AMD3100 groups. * <i>P</i><0.05 and ** <i>P</i><0.01.</p>
2014-02-24 03:32:53
Anatomy and physiology
cardiovascular system
Circulatory physiology
Model organisms
Animal models
rat
Molecular cell biology
Cellular types
Endothelial cells
cardiovascular
Pulmonary vascular diseases
Vascular biology
apoptosis
lungs
amd3100-treated
hypoxia