Proliferation and apoptosis in the lungs of AMD3100-treated SU5416/chronic hypoxia (SuHx) animals. Daniela Farkas Donatas Kraskauskas Jennifer I. Drake Aysar A. Alhussaini Vita Kraskauskiene Harm J. Bogaard Carlyne D. Cool Norbert F. Voelkel Laszlo Farkas 10.1371/journal.pone.0089810.g007 https://plos.figshare.com/articles/figure/_Proliferation_and_apoptosis_in_the_lungs_of_AMD3100_treated_SU5416_chronic_hypoxia_SuHx_animals_/943324 <p>(<b>A</b>) Representative Western blot for proliferating cell nuclear antigen (PCNA) and cleaved caspase-3 in the lung tissue protein lysate of naïve control animals (n = 3), SuHx + vehicle (n = 6) and SuHx + AMD3100 treated rats (n = 6). β-actin was used as loading control. (<b>B-C</b>) Densitometric analysis indicates increased PCNA (B) and cleaved caspase-3 (C) protein levels in the lungs of SuHx + vehicle animals vs. controls, and that AMD3100 treatment significantly reduced PCNA and cleaved caspase-3 protein levels in the lungs of SuHx animals. Densitometric values were normalized vs. β-actin and expressed as n-fold of naïve controls. n = 3 animals per group for controls and n = 6 animals per group for SuHx + vehicle and SuHx + AMD3100 groups. * <i>P</i><0.05 and ** <i>P</i><0.01.</p> 2014-02-24 03:32:53 Anatomy and physiology cardiovascular system Circulatory physiology Model organisms Animal models rat Molecular cell biology Cellular types Endothelial cells cardiovascular Pulmonary vascular diseases Vascular biology apoptosis lungs amd3100-treated hypoxia