10.6084/m9.figshare.94045.v1
Jean Lin
Jean
Lin
Leo Chen
Leo
Chen
Lawrence P Kane
Lawrence
P Kane
Imaging Tim-1 movement in response to latex beads
f1000research.com
2012
Cell Tracker Blue (blue)
confocal microscope
Jurkat T cells
Tim-1
ZAP70-TagRFP
Tim-1-GFP anti-OKT3
anti-CD28
Immunology
2012-08-10 10:47:40
Media
https://f1000.figshare.com/articles/media/Imaging_Tim-1_movement_in_response_to_latex_beads_/94045
<p>Jurkat T cells transiently co-transfected with Tim-1-GFP (green) and ZAP70-TagRFP (red) were incubated with anti-OKT3, anti-CD28 coated latex beads. Mid-plane images were taken every 23 seconds over the course of 19 minutes in a heated chamber on an Olympus FluoView 1000 confocal microscope.</p>