10.6084/m9.figshare.94045.v1 Jean Lin Jean Lin Leo Chen Leo Chen Lawrence P Kane Lawrence P Kane Imaging Tim-1 movement in response to latex beads f1000research.com 2012 Cell Tracker Blue (blue) confocal microscope Jurkat T cells Tim-1 ZAP70-TagRFP Tim-1-GFP anti-OKT3 anti-CD28 Immunology 2012-08-10 10:47:40 Media https://f1000.figshare.com/articles/media/Imaging_Tim-1_movement_in_response_to_latex_beads_/94045 <p>Jurkat T cells transiently co-transfected with Tim-1-GFP (green) and ZAP70-TagRFP (red) were incubated with anti-OKT3, anti-CD28 coated latex beads. Mid-plane images were taken every 23 seconds over the course of 19 minutes in a heated chamber on an Olympus FluoView 1000 confocal microscope.</p>