TY - DATA T1 - WT Tim-1 moves away from the nascent IS after APC stimulation PY - 2012/08/10 AU - Jean Lin AU - Leo Chen AU - Lawrence P Kane UR - https://f1000.figshare.com/articles/media/WT_Tim-1_moves_away_from_the_nascent_IS_after_APC_stimulation_/94044 DO - 10.6084/m9.figshare.94044.v1 L4 - https://ndownloader.figshare.com/files/96138 KW - Tim-1 KW - Cell Tracker Blue (blue) KW - Jurkat T cells KW - confocal microscope KW - Tim-1-GFP KW - Raji cells KW - APC stimulation KW - ZAP70-TagRFP KW - Immunology N2 - Jurkat T cells transiently transfected with Tim-1-GFP (green) and ZAP70-TagRFP (red) were incubated with Raji cells incubated with 1 mg/mL SEE and stained with Cell Tracker Blue (blue). Mid-plane images were taken every 6.7 seconds over the course of 19 minutes in a heated chamber on an Olympus FluoView 1000 confocal microscope. ER -