10.1371/journal.pcbi.1007252.g006 Alexey Uvarovskii Alexey Uvarovskii Isabel S. Naarmann-de Vries Isabel S. Naarmann-de Vries Christoph Dieterich Christoph Dieterich Application to experimental data from the MCF-7 pulse labeling time course experiment. Public Library of Science 2019 nucleotide conversion degradation rate estimates separation sequencing RNA 2019-08-07 17:33:50 Figure https://plos.figshare.com/articles/figure/Application_to_experimental_data_from_the_MCF-7_pulse_labeling_time_course_experiment_/9337391 <p><b>A</b>: We plot the diagonal term of the FIM computated at estimated parameter values and multiplied by , , to illustrate contributions from labeled and unlabeled fractions to estimations of degradation rates for different experimental points (MCF-7 experiment, 2, 4, and 8 hr) and fractions (labeled and unlabeled). The black lines are the limiting values for the according to <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1007252#pcbi.1007252.e064" target="_blank">Eq 20</a>. <b>B</b>: The modified FIM term is computed for a range of labeling times for one of the fastest (at the 0.1% quantile) and one of the slowest (at the 99,9% quantile) genes (<i>δ</i><sub>fast</sub> = 0.79hr<sup>−1</sup>, <i>δ</i><sub>slow</sub> = 0.019hr<sup>−1</sup>). The normalization coefficient for the labeled and unlabeled fractions is adjusted in such a way that their sequencing depth (total mean read count) at time <i>t</i> equals the sequencing depth of the total sample.</p>