Edg-1 is involved in RTEF-1 driven angiogenesis in endothelial cells. Ping He Melissa J. Philbrick Xiaojin An Jiaping Wu Angela F. Messmer-Blust Jian Li 10.1371/journal.pone.0088143.g003 https://plos.figshare.com/articles/figure/_Edg_1_is_involved_in_RTEF_1_driven_angiogenesis_in_endothelial_cells_/929323 <p><b>A.</b> Edg-1 expression was knocked down by Edg-1 siRNA. (** = <i>p</i><0.01, <i>vs</i> control siRNA treated RTEF-1 o/e HMEC-1) <b>B.</b> Edg-1 siRNA inhibited RTEF-1 driven endothelial cell aggregation. (# = <i>p</i><0.05, <i>vs</i> negative control siRNA treated control HMEC-1; ** = <i>p</i><0.01, <i>vs</i> negative control siRNA treated RTEF-1 o/e HMEC-1; ## = <i>p</i><0.01, <i>vs</i> negative control siRNA treated control HMEC-1; Triangle: aggregation area; Arrow: branch) <b>C.</b> Edg-1 siRNA inhibited RTEF-1 driven endothelial cell migration. (# = <i>p</i><0.05, <i>vs</i> negative control siRNA treated control HMEC-1; * = <i>p</i><0.05, <i>vs</i> negative control siRNA treated RTEF-1 o/e HMEC-1) <b>D.</b> Treatment with Edg-1 siRNA attenuated cell growth in RTEF-1 o/e HMEC-1 (* = <i>p</i><0.05; ** = <i>p</i><0.01, <i>vs</i> negative control siRNA). The results were quantified based on three experiments and are presented as mean±S.D.</p> 2014-02-10 03:22:39 genetics gene expression Gene function Molecular cell biology DNA transcription RNA interference Signal transduction Signaling in cellular processes G-protein signaling Cell growth cardiovascular Vascular biology rtef-1 driven angiogenesis endothelial