WNT7B increases osteoblast number and activity.
Jianquan Chen
Xiaolin Tu
Emel Esen
Kyu Sang Joeng
Congxin Lin
Jeffrey M. Arbeit
Markus A. Rüegg
Michael N. Hall
Liang Ma
Fanxin Long
10.1371/journal.pgen.1004145.g002
https://plos.figshare.com/articles/figure/_WNT7B_increases_osteoblast_number_and_activity_/918752
<p>(A, B) H&E staining of longitudinal tibia sections from two-month-old control (A) or <i>ColI-Wnt7b</i> littermates (B). 1°, 2°: primary and secondary ossification center. Shown to the right are higher magnification images of secondary ossification center (A1, B1), growth plate (A2, B2), primary spongiosa (A3, B3), and marrow region (A4, B4). Scale bar: 0.5 mm in panels A, B; 0.1 mm in panels A1–A4, B1–B4. (C) Serum osteocalcin levels of control (C) and <i>ColI-Wnt7b</i> littermates (7b) at one and two months of age. (D) Number of osteoblasts normalized to trabecular bone perimeter on longitudinal tibia sections. (E–F) Representative images of calcein double labeling in the humerus of two-month-old control (E) and <i>ColI-Wnt7b</i> (F) littermates. (G) Dynamic histomorphometry parameters from secondary ossification center of the humerus. MAR: mineral apposite rate; MS/BS: mineralizing surface over bone surface; BFR/BS: bone formation rate. (H) Serum CTX-I levels. (I) Osteoclast parameters from histomorphometry. #Oc/mm: osteoclast number normalized to trabecular bone perimeter, µm/Oc (average osteoclast surface), Oc S/BS (osteoclast surface normalized to bone surface). All bar graphs show mean ± STDEV, *: P<0.05, n = 3.</p>
2014-01-30 02:47:09
developmental biology
genetics
osteoblast