10.1371/journal.pone.0087776.g001 Evangeline W. Cornwell Evangeline W. Cornwell Azadeh Mirbod Azadeh Mirbod Chia-Ling Wu Chia-Ling Wu Susan C. Kandarian Susan C. Kandarian Robert W. Jackman Robert W. Jackman Effects of d.n. IKKβ-EGFP on muscle fiber cross sectional area (CSA). Public Library of Science 2014 Anatomy and physiology Musculoskeletal system muscle Muscle biochemistry Computational biology genomics Genome analysis tools Gene ontologies Genome expression analysis Genome sequencing microarrays Signaling networks Molecular cell biology Signal transduction Signaling cascades Protein kinase signaling cascade Signaling in cellular processes Nuclear signaling sectional 2014-01-29 04:09:07 Figure https://plos.figshare.com/articles/figure/_Effects_of_d_n_IKK_946_EGFP_on_muscle_fiber_cross_sectional_area_CSA_/918289 <p>(A) Mean tibialis anterior (TA) fiber cross-sectional area in control and C26 tumor-bearing mice in the presence or absence of d.n. IKKβ-EGFP. Each bar represents mean fiber area from 8 muscles (± SE). The average number of fibers measured per muscle was 200. (B) Fiber area frequency distribution of all fibers from muscles of control, C26, and C26 + d.n. IKKβ-EGFP groups. (C) Western blots of lysates from TA muscles injected with the empty vector (EV) or d.n. IKKβ-EGFP plasmid confirms overexpression of the fusion protein. d.n. IKKβ-EGFP is 114 kDa. All samples are from the same immunoblot. (D) Representative cross sections of TA muscles from control and C26 tumor-bearing mice injected with the d.n. IKKβ-EGFP plasmid. Fluorescent red represents laminin staining (anti-laminin incubation followed by Texas Red-X fluorescent dye-conjugated secondary antibody). Fluorescent green cytoplasm represents expression of transfected d.n. IKKβ-EGFP. C  =  control; * significantly different from control (<i>P</i><0.05). † significantly different from C26 fibers not transfected with d.n. IKKβ-EGFP (<i>P</i><0.05).</p>