10.1371/journal.pone.0087776.g001
Evangeline W. Cornwell
Evangeline
W. Cornwell
Azadeh Mirbod
Azadeh
Mirbod
Chia-Ling Wu
Chia-Ling
Wu
Susan C. Kandarian
Susan
C. Kandarian
Robert W. Jackman
Robert
W. Jackman
Effects of d.n. IKKβ-EGFP on muscle fiber cross sectional area (CSA).
Public Library of Science
2014
Anatomy and physiology
Musculoskeletal system
muscle
Muscle biochemistry
Computational biology
genomics
Genome analysis tools
Gene ontologies
Genome expression analysis
Genome sequencing
microarrays
Signaling networks
Molecular cell biology
Signal transduction
Signaling cascades
Protein kinase signaling cascade
Signaling in cellular processes
Nuclear signaling
sectional
2014-01-29 04:09:07
Figure
https://plos.figshare.com/articles/figure/_Effects_of_d_n_IKK_946_EGFP_on_muscle_fiber_cross_sectional_area_CSA_/918289
<p>(A) Mean tibialis anterior (TA) fiber cross-sectional area in control and C26 tumor-bearing mice in the presence or absence of d.n. IKKβ-EGFP. Each bar represents mean fiber area from 8 muscles (± SE). The average number of fibers measured per muscle was 200. (B) Fiber area frequency distribution of all fibers from muscles of control, C26, and C26 + d.n. IKKβ-EGFP groups. (C) Western blots of lysates from TA muscles injected with the empty vector (EV) or d.n. IKKβ-EGFP plasmid confirms overexpression of the fusion protein. d.n. IKKβ-EGFP is 114 kDa. All samples are from the same immunoblot. (D) Representative cross sections of TA muscles from control and C26 tumor-bearing mice injected with the d.n. IKKβ-EGFP plasmid. Fluorescent red represents laminin staining (anti-laminin incubation followed by Texas Red-X fluorescent dye-conjugated secondary antibody). Fluorescent green cytoplasm represents expression of transfected d.n. IKKβ-EGFP. C = control; * significantly different from control (<i>P</i><0.05). † significantly different from C26 fibers not transfected with d.n. IKKβ-EGFP (<i>P</i><0.05).</p>