10.1371/journal.pone.0086717.g002 Jessamine P. Winer-Jones Jessamine P. Winer-Jones Behrad Vahidi Behrad Vahidi Norma Arquilevich Norma Arquilevich Cong Fang Cong Fang Samuel Ferguson Samuel Ferguson Darren Harkins Darren Harkins Cory Hill Cory Hill Erich Klem Erich Klem Paul C. Pagano Paul C. Pagano Chrissy Peasley Chrissy Peasley Juan Romero Juan Romero Robert Shartle Robert Shartle Robert C. Vasko Robert C. Vasko William M. Strauss William M. Strauss Paul W. Dempsey Paul W. Dempsey CTC Flow cell operation and performance. Public Library of Science 2014 biotechnology genetics Cancer genetics Molecular cell biology cytometry flow cytometry Diagnostic medicine Test evaluation oncology Basic cancer research metastasis Cancer detection and diagnosis Cancer screening Cancer treatment Cancers and neoplasms 2014-01-29 03:42:30 Figure https://plos.figshare.com/articles/figure/_CTC_Flow_cell_operation_and_performance_/917894 <p>(A) Flow cytometry histograms of H1650, BT20, H1419, SK-Mel-28 and MCF7 cells labeled with 1 ug/mL of anti Trop2, Muc1, Her2, MelCAM and EpCAM antibodies respectively. Control antibody threshold is indicated by the red line (B) Recovery and purity of target cells using magnetophoretic capture from NHD blood: 90 cells/mL were spiked into NHD blood and purified on the LiquidBiopsy platform. Recovered cells were enumerated in the flow cell. Graphs show average recovery and purity of target cells +/−1 SD from 4 experiments for H1650, BT20, H1419 and SK-Mel-28 capture and 100 experiments for MCF7 capture.</p>